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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
27/06/2017 |
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Actualizado : |
27/06/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LANDI, L.; GÓMEZ, D.; BRACCINI, C.; PEREYRA, V.; SMITH, S.; MARVALDI, A. |
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Afiliación : |
LUCAS LANDI, Instituto Nacional de Tecnología Agropecuaria, Instituto de Recursos Biologicos, Bs.As. (Argentina); DEMIAN FERNANDO GOMEZ DAMIANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CECILIA L. BRACCINI, Instituto Nacional de Tecnología Agropecuaria, Instituto de Recursos Biologicos, Bs.As. (Argentina); VANINA A. PEREYRA, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Bs. As. (Argentina); SARAH M. SMITH, Michigan State University; ADRIANA E. MARVALDI, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Bs. As. (Argentina); CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas). |
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Título : |
Morphological and Molecular Identification of the Invasive Xylosandrus crassiusculus (Coleoptera: Curculionidae: Scolytinae) and Its South American Range Extending Into Argentina and Uruguay (Research) |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Annals of the Entomological Society of America, 2017, v.110 (3), p. 344-349. |
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ISSN : |
0013-8746 |
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DOI : |
10.1093/aesa/sax032 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 26 October 2016; Accepted: 04 January 2017; Published: 08 March 2017 |
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Contenido : |
ABSTRACT.
The occurrence of the exotic "granulate ambrosia beetle" Xylosandrus crassiusculus (Motschulsky) in Argentina and Uruguay is reported for the first time, documenting expansion in South America. Morphological characters are provided to allow distinction of this species from other similar ambrosia beetles. Molecular identification was also done on the basis of the 50 region of the mitochondrial cytochrome c oxidase I (COI) gene, obtained from a specimen from Argentina and compared with available "barcode" sequences in public databases. Phylogenetic analyses, via maximum likelihood and parsimony, were performed using COI sequences from 17 terminals, including 14 sequences of X. crassiusculus from different continents plus other Xylosandrus and Cnestus species as outgroups. Results from the sequence analyses confirm the identity of X. crassiusculus. The specimen from Argentina and the two sampled from United States showed no differences in their COI sequences. Further studies are needed, on the genetic variation through the native and introduced ranges of the granulate ambrosia beetle, to ascertain the source/s of origin of this biological invasion in the Americas. The present paper reports the expansion of this exotic species in South America into Argentina and Uruguay.
© The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. |
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Palabras claves : |
AMBROSIA BEETLE; DNA; EXOTIC SPECIES; XYLEBORINI. |
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Thesagro : |
COLEOPTERA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02401naa a2200277 a 4500
001 1057303
005 2017-06-27
008 2017 bl uuuu u00u1 u #d
022 $a0013-8746
024 7 $a10.1093/aesa/sax032$2DOI
100 1 $aLANDI, L.
245 $aMorphological and Molecular Identification of the Invasive Xylosandrus crassiusculus (Coleoptera$bCurculionidae: Scolytinae) and Its South American Range Extending Into Argentina and Uruguay (Research)$h[electronic resource]
260 $c2017
500 $aArticle history: Received: 26 October 2016; Accepted: 04 January 2017; Published: 08 March 2017
520 $aABSTRACT. The occurrence of the exotic "granulate ambrosia beetle" Xylosandrus crassiusculus (Motschulsky) in Argentina and Uruguay is reported for the first time, documenting expansion in South America. Morphological characters are provided to allow distinction of this species from other similar ambrosia beetles. Molecular identification was also done on the basis of the 50 region of the mitochondrial cytochrome c oxidase I (COI) gene, obtained from a specimen from Argentina and compared with available "barcode" sequences in public databases. Phylogenetic analyses, via maximum likelihood and parsimony, were performed using COI sequences from 17 terminals, including 14 sequences of X. crassiusculus from different continents plus other Xylosandrus and Cnestus species as outgroups. Results from the sequence analyses confirm the identity of X. crassiusculus. The specimen from Argentina and the two sampled from United States showed no differences in their COI sequences. Further studies are needed, on the genetic variation through the native and introduced ranges of the granulate ambrosia beetle, to ascertain the source/s of origin of this biological invasion in the Americas. The present paper reports the expansion of this exotic species in South America into Argentina and Uruguay. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.
650 $aCOLEOPTERA
653 $aAMBROSIA BEETLE
653 $aDNA
653 $aEXOTIC SPECIES
653 $aXYLEBORINI
700 1 $aGÓMEZ, D.
700 1 $aBRACCINI, C.
700 1 $aPEREYRA, V.
700 1 $aSMITH, S.
700 1 $aMARVALDI, A.
773 $tAnnals of the Entomological Society of America, 2017$gv.110 (3), p. 344-349.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/02/2021 |
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Actualizado : |
22/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MAESO, D.; FEDERICI, M.; MARTÍNEZ, A.; SILVERA, M.; GONCALVEZ, L. |
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Afiliación : |
DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A. MARTÍNEZ, Facultad de Ciencias, Universidad de la Repu?blica, Montevideo, Uruguay.; MARIANA SILVERA ORREGO, Facultad de Ciencias, Universidad de la Repu?blica, Montevideo, Uruguay.; ANA LUCIA GONCALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Studies on pear decline disease in Uruguay. [Conference paper]. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Acta Horticulturae, February 2021, N°1303, p. 343-350. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.48 |
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ISSN : |
0567-7572 (print); 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2021.1303.48 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. |
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Contenido : |
Abstract:
Pear decline (PD) caused by 'Candidatus Phytoplasma pyri' (subgroup 16SrX-C of apple proliferation or AP group of phytoplasmas) is an important pear disease (Pyrus communis L.). Pear production in Uruguay is based on 'Williams' plants initially grafted on quince that are planted deep and thus transformed into scion-rooted trees. Less vigorous rootstocks like Pyrus spp. and quinces that could be more susceptible to decline are beginning to be used for precocity. Surveys were conducted on plants grafted on different rootstocks to know if PD was present in the pear growing area of Uruguay (southern part near Montevideo city) by searching for premature reddening, upward rolling of leaves and decline. Phytoplasmas were observed in 35 of 70 samples using the DAPI (4',6-diamidino-2-fenilindol) staining test. Ten positive and 10 negative samples were analyzed by polymerase chain reaction (PCR) assay using AP-group specific primers f01/r01. A 930 bp amplicon was obtained only from all DAPI positive samples. The f01/r01 amplicon was sequenced and showed 100% identity with the 16S rRNA gene sequence of PD phytoplasma in the NCBI database. A relationship was observed between phytoplasma detection, reddening, psylla infestation and premature leaf fall in this survey in 1995. Presence of PD-symptoms and phytoplasma detection by PCR were also evaluated in an experiment where 20 pear scion/rootstock combinations were compared during 2005-2011. Plants of self-rooted 'Williams' or grafted onto OH×F 40 and OH×F 69 stocks showed less early reddening and leaf fall than plants grafted onto quince rootstocks. Phytoplasma detection in this trial varied among seasons and was not associated with the presence of symptoms. Phytoplasmas were detected in pear psylla insects during the whole 2009-2011 seasons in four pear orchards. An association between the effectiveness of psylla control, phytoplasma detection and early reddening and leaf fall was found in an experiment with traditional 'Williams' self-rooted plants in 2011. These results indicate the presence of PD phytoplasma in Uruguayan pear orchards and in psylla insects during the whole season.
@ International Society for Horticultural Science. MenosAbstract:
Pear decline (PD) caused by 'Candidatus Phytoplasma pyri' (subgroup 16SrX-C of apple proliferation or AP group of phytoplasmas) is an important pear disease (Pyrus communis L.). Pear production in Uruguay is based on 'Williams' plants initially grafted on quince that are planted deep and thus transformed into scion-rooted trees. Less vigorous rootstocks like Pyrus spp. and quinces that could be more susceptible to decline are beginning to be used for precocity. Surveys were conducted on plants grafted on different rootstocks to know if PD was present in the pear growing area of Uruguay (southern part near Montevideo city) by searching for premature reddening, upward rolling of leaves and decline. Phytoplasmas were observed in 35 of 70 samples using the DAPI (4',6-diamidino-2-fenilindol) staining test. Ten positive and 10 negative samples were analyzed by polymerase chain reaction (PCR) assay using AP-group specific primers f01/r01. A 930 bp amplicon was obtained only from all DAPI positive samples. The f01/r01 amplicon was sequenced and showed 100% identity with the 16S rRNA gene sequence of PD phytoplasma in the NCBI database. A relationship was observed between phytoplasma detection, reddening, psylla infestation and premature leaf fall in this survey in 1995. Presence of PD-symptoms and phytoplasma detection by PCR were also evaluated in an experiment where 20 pear scion/rootstock combinations were compared during 2005-2011. Plants of self-rooted 'Williams' or g... Presentar Todo |
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Palabras claves : |
Cacopsylla bidens; Candidatus Phytoplasma pyri; European pear; Scion-rootstock affinity. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 03270naa a2200253 a 4500
001 1061745
005 2021-02-22
008 2021 bl uuuu u00u1 u #d
022 $a0567-7572 (print); 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2021.1303.48$2DOI
100 1 $aMAESO, D.
245 $aStudies on pear decline disease in Uruguay. [Conference paper].$h[electronic resource]
260 $c2021
500 $aArticle history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein.
520 $aAbstract: Pear decline (PD) caused by 'Candidatus Phytoplasma pyri' (subgroup 16SrX-C of apple proliferation or AP group of phytoplasmas) is an important pear disease (Pyrus communis L.). Pear production in Uruguay is based on 'Williams' plants initially grafted on quince that are planted deep and thus transformed into scion-rooted trees. Less vigorous rootstocks like Pyrus spp. and quinces that could be more susceptible to decline are beginning to be used for precocity. Surveys were conducted on plants grafted on different rootstocks to know if PD was present in the pear growing area of Uruguay (southern part near Montevideo city) by searching for premature reddening, upward rolling of leaves and decline. Phytoplasmas were observed in 35 of 70 samples using the DAPI (4',6-diamidino-2-fenilindol) staining test. Ten positive and 10 negative samples were analyzed by polymerase chain reaction (PCR) assay using AP-group specific primers f01/r01. A 930 bp amplicon was obtained only from all DAPI positive samples. The f01/r01 amplicon was sequenced and showed 100% identity with the 16S rRNA gene sequence of PD phytoplasma in the NCBI database. A relationship was observed between phytoplasma detection, reddening, psylla infestation and premature leaf fall in this survey in 1995. Presence of PD-symptoms and phytoplasma detection by PCR were also evaluated in an experiment where 20 pear scion/rootstock combinations were compared during 2005-2011. Plants of self-rooted 'Williams' or grafted onto OH×F 40 and OH×F 69 stocks showed less early reddening and leaf fall than plants grafted onto quince rootstocks. Phytoplasma detection in this trial varied among seasons and was not associated with the presence of symptoms. Phytoplasmas were detected in pear psylla insects during the whole 2009-2011 seasons in four pear orchards. An association between the effectiveness of psylla control, phytoplasma detection and early reddening and leaf fall was found in an experiment with traditional 'Williams' self-rooted plants in 2011. These results indicate the presence of PD phytoplasma in Uruguayan pear orchards and in psylla insects during the whole season. @ International Society for Horticultural Science.
653 $aCacopsylla bidens
653 $aCandidatus Phytoplasma pyri
653 $aEuropean pear
653 $aScion-rootstock affinity
700 1 $aFEDERICI, M.
700 1 $aMARTÍNEZ, A.
700 1 $aSILVERA, M.
700 1 $aGONCALVEZ, L.
773 $tActa Horticulturae, February 2021, N°1303, p. 343-350. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.48
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